Microtiter wells coated with recombinant HBsAg (adw and adr), are exposed to test specimens, which may contain mouse IgG reactive antibodies. After an incubation period, unbound components in the test sample are washed away. Specific bound mouse IgG react with an anti-mouse IgG conjugated with HRP during a second incubation period. Following a second wash cycle, specific bound enzyme conjugate is detected by reaction with the substrate solution, 2,2' Azino-di[3-ethyl-benzthiazolinesulfonate (ABTS). The assay is measured spectrophotometrically to indicate the level of Mouse Anti-HbsAg antibodies present in a sample. *FOR RESEARCH USE ONLY*
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